Catalog Number - 68312-25P-4

Cogent RP C12™ is a unique reversed-phase HPLC stationary phase designed to provide balanced hydrophobic retention, rapid equilibration, and excellent reproducibility. Manufactured using a rigid adamantane-shaped carbon silane bonded to ultra-high purity silica and fully double end capped, this stationary phase offers selectivity that falls between traditional C8 and C18 phases. The distinctive ligand structure provides excellent performance for complex mixtures and applications requiring robust retention behavior across a wide range of mobile-phase conditions.

68312-25P-4 is an HPLC column packed with 3 µm RP C12™ particles featuring a 100 Å pore size in a 4.0 mm ID x 250 mm format. The 250 mm length provides maximum chromatographic resolution and peak capacity within the RP C12 product family, while the 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and system configuration.

Compatible with virtually all reversed-phase mobile phases, including 100% aqueous conditions, this column maintains consistent retention without the phase collapse often associated with some traditional reversed-phase columns. Retention is typically greater than C8 phases but lower than C18 phases for the same hydrophobic compounds. The extended 250 mm length makes this column particularly well suited for complex sample separations, impurity profiling, method development, and applications requiring the highest possible resolving power.

Key Benefits

• RP C12™ stationary phase

• Fully double end capped

• Adamantane-shaped bonded ligand

• Ultra-high purity silica support

• Compatible with 100% aqueous mobile phases

• Rapid equilibration between runs

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Maximum chromatographic resolution

• Retention characteristics between C8 and C18 phases

Typical Applications

• High-throughput screening (HTS)

• Reversed-phase HPLC

• Complex mixture separations

• Pharmaceutical analysis

• Method development

• Quality control testing

• Hydrophobic compound analysis

• Impurity profiling

• Gradient separations

• Research and development

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane Carbon Silane

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 4.0 mm ID x 250 mm

• Length: 250 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Special Order Item Information

• For complete details, please review the Special Order Item Information.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Compatible with 100% aqueous mobile phases

• Maintains consistent retention from run to run

• Rapid equilibration supports high-throughput workflows

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 250 mm length provides maximum resolution and peak capacity for demanding separations

• Retention is generally greater than C8 phases and less than C18 phases

• Excellent choice for complex sample separations and screening applications

• Well suited for impurity profiling and advanced method development

• Particularly useful when maximum chromatographic resolution is required

• This is a Special Order Item